IGEM:MIT/2006/Notebook/2006-8-29: Difference between revisions
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#create registry biobrick parts for inverter constructs (w/ and w/out osmY attached) '''as well as all intermediate BGD, IAGD, SAGD parts!!!!''' - p.s. anybody out of town is welcome to help. we seem to be very behind on this. | #'''UPDATE REGISTRY''': create registry biobrick parts for inverter constructs (w/ and w/out osmY attached) '''as well as all intermediate BGD, IAGD, SAGD parts!!!!''' - p.s. anybody out of town is welcome to help. we seem to be very behind on this. | ||
#try to track down/organize mystery tubes/glycerols using digest/ligation sheets | #try to track down/organize mystery tubes/glycerols using digest/ligation sheets | ||
#analyze sequencing results mid-morning | #analyze sequencing results mid-morning | ||
Revision as of 18:09, 28 August 2006
to do
- UPDATE REGISTRY: create registry biobrick parts for inverter constructs (w/ and w/out osmY attached) as well as all intermediate BGD, IAGD, SAGD parts!!!! - p.s. anybody out of town is welcome to help. we seem to be very behind on this.
- try to track down/organize mystery tubes/glycerols using digest/ligation sheets
- analyze sequencing results mid-morning
- then miniprep/sequence all new pchBA LCs that could still possibly have R0011 attached and throw out bad
- check for which osmY-Q-J.xxx structures look good and throw out bad
- glycerol pE3/pE3R (plus miniprep) and YYC912 IK strain
- make new LCs of correct constructs and label glycerols with biobrick names/numbers for updating our final bb-box
- make iGEM yeast LC (one big for pelleting/baking and one small for miniprepping/digesting/ligating)
- 3-part assemble/transform B0015 with the 2 correctly mutagenized 30.Bat2.30.THI3
- transform good osmY-Q-J.xxx into IKN cells
- add methyl salicylate to pseudomonas cells and see if it is readily degraded
