IGEM:MIT/2006/Notebook/2006-8-29: Difference between revisions

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==to do==
==to do==
#'''UPDATE REGISTRY''': create registry biobrick parts for inverter constructs (w/ and w/out osmY attached) '''as well as all intermediate BGD, IAGD, SAGD parts!!!!''' - p.s. anybody out of town is welcome to help. we seem to be very behind on this.
#analyze sequencing results mid-morning '''done'''
#analyze sequencing results mid-morning
#*then miniprep/sequence all new rbs.pchBA.term LCs that could still possibly have R0011 attached and throw out bad '''all failed to attach an rbs, so are starting over assembly from pchBA.Term-mut glycerol that we think is correct'''
#*then miniprep/sequence all new rbs.pchBA.term LCs that could still possibly have R0011 attached and throw out bad
#*check for which osmY-Q-J.xxx structures look good and throw out bad '''incomplete sequencing results, but O-Q-119B is good and potentially O-Q-199A'''
#*check for which osmY-Q-J.xxx structures look good and throw out bad
#glycerol pE3/pE3R (plus miniprep) and YYC912 IK strain '''done, but pE3 had a low miniprep concentration'''
#glycerol pE3/pE3R (plus miniprep) and YYC912 IK strain
#glycerol with nice labels, miniprep, sequence final bb-construct parts (6) to doublecheck their integrity '''done, but realized that the J45396 that I chose last night was not a mutagenized construct - made a new correct LC'''
#glycerol with nice labels, miniprep, sequence final bb-construct parts (6) to doublecheck their integrity
#make iGEM yeast LC (one big for pelleting/baking and one small for miniprepping/digesting/ligating) '''done, and bread machine has arrived'''
#make iGEM yeast LC (one big for pelleting/baking and one small for miniprepping/digesting/ligating)
#3-part assemble/transform B0015 with the 2 correctly mutagenized/ES digested 30.Bat2.30.THI3 '''done'''
#3-part assemble/transform B0015 with the 2 correctly mutagenized 30.Bat2.30.THI3
#add methyl salicylate to pseudomonas cells (if grown-up enough) and see if it is readily degraded '''done'''
#transform good osmY-Q-J.xxx into IKN cells
#add methyl salicylate to pseudomonas cells (if grown-up enough) and see if it is readily degraded


==time line==
==time line==

Revision as of 19:15, 29 August 2006

to do

  1. analyze sequencing results mid-morning done
    • then miniprep/sequence all new rbs.pchBA.term LCs that could still possibly have R0011 attached and throw out bad all failed to attach an rbs, so are starting over assembly from pchBA.Term-mut glycerol that we think is correct
    • check for which osmY-Q-J.xxx structures look good and throw out bad incomplete sequencing results, but O-Q-119B is good and potentially O-Q-199A
  2. glycerol pE3/pE3R (plus miniprep) and YYC912 IK strain done, but pE3 had a low miniprep concentration
  3. glycerol with nice labels, miniprep, sequence final bb-construct parts (6) to doublecheck their integrity done, but realized that the J45396 that I chose last night was not a mutagenized construct - made a new correct LC
  4. make iGEM yeast LC (one big for pelleting/baking and one small for miniprepping/digesting/ligating) done, and bread machine has arrived
  5. 3-part assemble/transform B0015 with the 2 correctly mutagenized/ES digested 30.Bat2.30.THI3 done
  6. add methyl salicylate to pseudomonas cells (if grown-up enough) and see if it is readily degraded done

time line

  1. first: make parts in registry for easier sequence analysis
  2. second: analyze sequencing
  3. third: minipreps and glycerols
  4. fourth: sequencing
  5. fifth: ligations/transformations
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