IGEM:MIT/2006/Notebook/2006-8-29: Difference between revisions
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==to do== | ==to do== | ||
#''' | #analyze sequencing results mid-morning '''- done''' | ||
#*then miniprep/sequence all new rbs.pchBA.term LCs that could still possibly have R0011 attached and throw out bad '''- all failed to attach an rbs, so are starting over assembly from pchBA.Term-mut glycerol that we think is correct''' | |||
#*then miniprep/sequence all new rbs.pchBA.term LCs that could still possibly have R0011 attached and throw out bad | #*check for which osmY-Q-J.xxx structures look good and throw out bad '''- incomplete sequencing results, but O-Q-119B is good and potentially O-Q-199A is too''' | ||
#*check for which osmY-Q-J.xxx structures look good and throw out bad | #glycerol pE3/pE3R (plus miniprep) and YYC912 IK strain '''- done, but pE3 had a low miniprep concentration''' | ||
#glycerol pE3/pE3R (plus miniprep) and YYC912 IK strain | #glycerol with nice labels, miniprep, sequence final bb-construct parts (6) to doublecheck their integrity '''- done, but realized that the J45396 is not a mutagenized construct - made a new correct LC''' | ||
#glycerol with nice labels, miniprep, sequence final bb-construct parts (6) to doublecheck their integrity | #make iGEM yeast LC (one big for pelleting/baking and one small for miniprepping/digesting/ligating) '''- done, and bread machine has arrived''' | ||
#make iGEM yeast LC (one big for pelleting/baking and one small for miniprepping/digesting/ligating) | #3-part assemble/transform B0015 with the 2 correctly mutagenized/ES digested 30.Bat2.30.THI3 '''- done''' | ||
#3-part assemble/transform B0015 with the 2 correctly mutagenized 30.Bat2.30.THI3 | #add methyl salicylate to pseudomonas cells (if grown-up enough) and see if it is readily degraded '''- done''' | ||
#add methyl salicylate to pseudomonas cells (if grown-up enough) and see if it is readily degraded | |||
==time line== | ==today's time line== | ||
#first: | #first: remove all LCs | ||
#second: analyze sequencing | #second: analyze sequencing | ||
#third: minipreps and glycerols | #third: minipreps and glycerols | ||
#fourth: sequencing | #fourth: sequencing | ||
#fifth: ligations/transformations | #fifth: ligations/transformations | ||
Latest revision as of 19:19, 29 August 2006
to do
- analyze sequencing results mid-morning - done
- then miniprep/sequence all new rbs.pchBA.term LCs that could still possibly have R0011 attached and throw out bad - all failed to attach an rbs, so are starting over assembly from pchBA.Term-mut glycerol that we think is correct
- check for which osmY-Q-J.xxx structures look good and throw out bad - incomplete sequencing results, but O-Q-119B is good and potentially O-Q-199A is too
- glycerol pE3/pE3R (plus miniprep) and YYC912 IK strain - done, but pE3 had a low miniprep concentration
- glycerol with nice labels, miniprep, sequence final bb-construct parts (6) to doublecheck their integrity - done, but realized that the J45396 is not a mutagenized construct - made a new correct LC
- make iGEM yeast LC (one big for pelleting/baking and one small for miniprepping/digesting/ligating) - done, and bread machine has arrived
- 3-part assemble/transform B0015 with the 2 correctly mutagenized/ES digested 30.Bat2.30.THI3 - done
- add methyl salicylate to pseudomonas cells (if grown-up enough) and see if it is readily degraded - done
today's time line
- first: remove all LCs
- second: analyze sequencing
- third: minipreps and glycerols
- fourth: sequencing
- fifth: ligations/transformations
