IGEM:MIT/2006/Notebook/2006-8-29

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Revision as of 16:46, 28 August 2006 by Skatebro (talk | contribs) (→‎to do)
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to do

  1. create registry biobrick parts for inverter constructs (w/ and w/out osmY attached)
  2. try to track down/organize mystery tubes/glycerols using digest/ligation sheets
  3. analyze sequencing results mid-morning
    • then miniprep/sequence all new pchBA LCs that could still possibly have R0011 attached and throw out bad
    • check for which osmY-Q-J.xxx structures look good and throw out bad
  4. glycerol pE3/pE3R (plus miniprep) and YYC912 IK strain
  5. make new LCs of correct constructs and label glycerols with biobrick names/numbers for updating our final bb-box
  6. make iGEM yeast LC (one big for pelleting/baking and one small for miniprepping/digesting/ligating)
  7. 3-part assemble/transform B0015 with the 2 correctly mutagenized 30.Bat2.30.THI3
  8. transform good osmY-Q-J.xxx into IKN cells
  9. add methyl salicylate to pseudomonas cells and see if it is readily degraded
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