IGEM:MIT/2006/Notebook/2006-8-3

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(to do)
(to do)
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#Miniprep BSMT.B0015 LC  
#Miniprep BSMT.B0015 LC  
#Cut BSMT.B0015 with XP
#Cut BSMT.B0015 with XP
 +
#Miniprep B0030
 +
#Cut B0030 with ES
 +
 +
#Gel extract BSMT.B0015<sub>XP</sub>
 +
#Gel extract B0030<sub>SP</sub> backbone
 +
#Ligate BSMT.B0015<sub>XP</sub> and B0030<sub>SP</sub> backbone-> transform
 +
 +
#Ligate B0030<sub>ES</sub> with BSMT.B0015<sub>XP</sub> in A/C<sub>EP</sub>
 +
#Ligate B0030<sub>ES</sub> with BSMT.B0015<sub>XP</sub> in A/T<sub>EP</sub>

Revision as of 17:01, 2 August 2006

to do

  1. test osmY-E0840 with plate reader
  2. Miniprep BSMT.B0015 LC
  3. Cut BSMT.B0015 with XP
  4. Miniprep B0030
  5. Cut B0030 with ES
  1. Gel extract BSMT.B0015XP
  2. Gel extract B0030SP backbone
  3. Ligate BSMT.B0015XP and B0030SP backbone-> transform
  1. Ligate B0030ES with BSMT.B0015XP in A/CEP
  2. Ligate B0030ES with BSMT.B0015XP in A/TEP
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