IGEM:MIT/2006/Notebook/2006-8-3

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(to do)
(to do)
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#Cut B0030 with ES
#Cut B0030 with ES
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==Ligations/Transformations To Do==
#Gel extract BSMT.B0015<sub>XP</sub>
#Gel extract BSMT.B0015<sub>XP</sub>
#Gel extract B0030<sub>SP</sub> backbone
#Gel extract B0030<sub>SP</sub> backbone
#Ligate BSMT.B0015<sub>XP</sub> and B0030<sub>SP</sub> backbone-> transform  
#Ligate BSMT.B0015<sub>XP</sub> and B0030<sub>SP</sub> backbone-> transform  
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+
<br>
#Ligate B0030<sub>ES</sub> with BSMT.B0015<sub>XP</sub> in A/C<sub>EP</sub>
#Ligate B0030<sub>ES</sub> with BSMT.B0015<sub>XP</sub> in A/C<sub>EP</sub>
#Ligate B0030<sub>ES</sub> with BSMT.B0015<sub>XP</sub> in A/T<sub>EP</sub>
#Ligate B0030<sub>ES</sub> with BSMT.B0015<sub>XP</sub> in A/T<sub>EP</sub>

Revision as of 16:01, 2 August 2006

to do

  1. test osmY-E0840 with plate reader
  2. Miniprep BSMT.B0015 LC
  3. Cut BSMT.B0015 with XP
  4. Miniprep B0030
  5. Cut B0030 with ES

Ligations/Transformations To Do

  1. Gel extract BSMT.B0015XP
  2. Gel extract B0030SP backbone
  3. Ligate BSMT.B0015XP and B0030SP backbone-> transform


  1. Ligate B0030ES with BSMT.B0015XP in A/CEP
  2. Ligate B0030ES with BSMT.B0015XP in A/TEP
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