IGEM:MIT/2006/Notebook/2006-8-3
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(Difference between revisions)
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#Cut B0030 with ES | #Cut B0030 with ES | ||
| + | ==Ligations/Transformations To Do== | ||
#Gel extract BSMT.B0015<sub>XP</sub> | #Gel extract BSMT.B0015<sub>XP</sub> | ||
#Gel extract B0030<sub>SP</sub> backbone | #Gel extract B0030<sub>SP</sub> backbone | ||
#Ligate BSMT.B0015<sub>XP</sub> and B0030<sub>SP</sub> backbone-> transform | #Ligate BSMT.B0015<sub>XP</sub> and B0030<sub>SP</sub> backbone-> transform | ||
| - | + | <br> | |
#Ligate B0030<sub>ES</sub> with BSMT.B0015<sub>XP</sub> in A/C<sub>EP</sub> | #Ligate B0030<sub>ES</sub> with BSMT.B0015<sub>XP</sub> in A/C<sub>EP</sub> | ||
#Ligate B0030<sub>ES</sub> with BSMT.B0015<sub>XP</sub> in A/T<sub>EP</sub> | #Ligate B0030<sub>ES</sub> with BSMT.B0015<sub>XP</sub> in A/T<sub>EP</sub> | ||
Revision as of 17:01, 2 August 2006
to do
- test osmY-E0840 with plate reader
- Miniprep BSMT.B0015 LC
- Cut BSMT.B0015 with XP
- Miniprep B0030
- Cut B0030 with ES
Ligations/Transformations To Do
- Gel extract BSMT.B0015XP
- Gel extract B0030SP backbone
- Ligate BSMT.B0015XP and B0030SP backbone-> transform
- Ligate B0030ES with BSMT.B0015XP in A/CEP
- Ligate B0030ES with BSMT.B0015XP in A/TEP
