IGEM:MIT/2006/Notebook/2006-8-3

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(to do)
Line 4: Line 4:
#Cut BSMT.B0015 with XP
#Cut BSMT.B0015 with XP
#Miniprep B0030  
#Miniprep B0030  
-
#Cut B0030 with ES
+
#Cut B0030 with ES and SP(bkb)
 +
#Miniprep R0040
 +
#Cut R0040 with ES and SP(bkb)
==WG Ligations/Transformations To Do==
==WG Ligations/Transformations To Do==

Revision as of 17:08, 2 August 2006

to do

  1. test osmY-E0840 with plate reader
  2. Miniprep BSMT.B0015 LC
  3. Cut BSMT.B0015 with XP
  4. Miniprep B0030
  5. Cut B0030 with ES and SP(bkb)
  6. Miniprep R0040
  7. Cut R0040 with ES and SP(bkb)

WG Ligations/Transformations To Do

  1. Gel extract BSMT.B0015XP
  2. Gel extract B0030SP backbone
  3. Ligate BSMT.B0015XP and B0030SP backbone-> transform


  1. Ligate B0030ES with BSMT.B0015XP in A/CEP
  2. Ligate B0030ES with BSMT.B0015XP in A/TEP

osmY Ligations/Transformations To Do

  1. osmY and A/CEP and A/TEP
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