IGEM:MIT/2006/Notebook/2006-8-3: Difference between revisions
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==ATF1 Ligation/Transformation To Do== | ==ATF1 Ligation/Transformation To Do== | ||
#Ligate ATF1.B0015<sub>XP</sub> with B0030<sub>ES</sub> in A/C<sub>EP</sub> and A/T<sub>ESP</sub> | #Ligate ATF1.B0015<sub>XP</sub> with B0030<sub>ES</sub> in A/C<sub>EP</sub> and A/T<sub>ESP</sub> | ||
==IAA Digest== | |||
#BAT2 with EP, ligate with A/C and A/T<sub>EP</sub> | |||
#THI3 with SX | |||
#BKB with SX | |||
Revision as of 14:44, 2 August 2006
Primer To Do
- Order new pchBA primers
Miniprep/Digest To Do
- test osmY-E0840 with plate reader
- Miniprep BSMT.B0015 LC
- Cut BSMT.B0015 with XP
- Miniprep B0030
- Cut B0030 with ES and SP(bkb)
- Miniprep R0040
- Cut R0040 with ES and SP(bkb)
WG Ligations/Transformations To Do
- Gel extract BSMT.B0015XP
- Gel extract B0030SP backbone
- Ligate BSMT.B0015XP and B0030SP backbone-> transform
- Ligate B0030ES with BSMT.B0015XP in A/CEP
- Ligate B0030ES with BSMT.B0015XP in A/TEP
osmY Ligations/Transformations To Do
- osmY and A/CEP and A/TEP
ATF1 Miniprep/Digest To Do
- Miniprep ATF1.B0015 LC
- Cut ATF1.B0015 with XP
- Gel extract some of ATF1.B0015XP
ATF1 Ligation/Transformation To Do
- Ligate ATF1.B0015XP with B0030ES in A/CEP and A/TESP
IAA Digest
- BAT2 with EP, ligate with A/C and A/TEP
- THI3 with SX
- BKB with SX
