IGEM:MIT/2006/Notebook/2006-8-3
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==WG Ligations/Transformations To Do== | ==WG Ligations/Transformations To Do== | ||
#Gel extract BSMT.B0015<sub>XP</sub> | #Gel extract BSMT.B0015<sub>XP</sub> | ||
| - | # | + | #PCR Cleanup B0030<sub>SP</sub> backbone |
#Ligate BSMT.B0015<sub>XP</sub> and B0030<sub>SP</sub> backbone-> transform | #Ligate BSMT.B0015<sub>XP</sub> and B0030<sub>SP</sub> backbone-> transform | ||
<br> | <br> | ||
Revision as of 17:59, 2 August 2006
Contents |
Primer To Do
- Order new pchBA primers
Miniprep/Digest To Do
- test osmY-E0840 with plate reader
- Miniprep BSMT.B0015 LC
- Cut BSMT.B0015 with XP
- Miniprep B0030
- Cut B0030 with ES and SP(bkb)
- Miniprep R0040
- Cut R0040 with ES and SP(bkb)
WG Ligations/Transformations To Do
- Gel extract BSMT.B0015XP
- PCR Cleanup B0030SP backbone
- Ligate BSMT.B0015XP and B0030SP backbone-> transform
- Ligate B0030ES with BSMT.B0015XP in A/CEP
- Ligate B0030ES with BSMT.B0015XP in A/TEP
osmY Ligations/Transformations To Do
- osmY and A/CEP and A/TEP
ATF1 Miniprep/Digest To Do
- Miniprep ATF1.B0015 LC
- Cut ATF1.B0015 with XP
- Gel extract some of ATF1.B0015XP
ATF1 Ligation/Transformation To Do
- Ligate ATF1.B0015XP with B0030ES in A/CEP and A/TESP
IAA Digest
- BAT2 with EP, ligate with A/C and A/TEP
- THI3 with SX
- BKB with SX
IAA Ligations
- BAT2EP w/ A/C & A/T BKB
- THI3SX w/ psBIAK3SX->A/K plate
