IGEM:MIT/2006/Notebook/2006-8-3

Miniprep/Digest To Do

1. test osmY-E0840 with plate reader
2. Miniprep BSMT.B0015 LC
3. Cut BSMT.B0015 with XP
4. Miniprep B0030
5. Cut B0030 with ES and SP(bkb)
6. Miniprep R0040
7. Cut R0040 with ES and SP(bkb)

WG Ligations/Transformations To Do

1. Gel extract BSMT.B0015_XP
2. Gel extract B0030_SP backbone
3. Ligate BSMT.B0015_XP and B0030_SP backbone-> transform

1. Ligate B0030_ES with BSMT.B0015_XP in A/C_EP
2. Ligate B0030_ES with BSMT.B0015_XP in A/T_EP

osmY Ligations/Transformations To Do

1. osmY and A/C_EP and A/T_EP