IGEM:MIT/2006/Notebook/2006-8-30: Difference between revisions

Revision as of 19:41, 29 August 2006

UPDATE REGISTRY: create registry biobrick parts and choose numbers for inverter constructs (w/ and w/out osmY attached) as well as all intermediate BGD, IAGD, SAGD parts!!!! - p.s. anybody out of town is welcome to help. we seem to be very behind on this.
digest J45298 miniprep (from 8/29) with XP (run a gel to make sure it looks correctly mutagenized)
miniprep iGEM yeast shuttle vector and digest it appropriately (check the MCS, or Veena took notes and might know). Also digest our BGD (J45200,J45220) appropriately to insert at the MCS
primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29)
check if the 0-Q-199A forward sequencing is in from the re-run and see if it looks good
glycerol/miniprep/sequence J45396
glycerol new Q119
pellet and resuspend yeast cells in milk and find a good bread recipe
3-part assemble rbss to pchBA.15-muts and transform into top10
put our BGD into yeast shuttle vector and transform into top10
consider transforming O-Q-119 and O-Q-199 (?) into IK to smell test
pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs
LC whatever we need for a osmY-Q0440-E0840 plate reader test (and sign up for the block)

@@ Line 1: / Line 1: @@
 ==to do==
-#try to PCR pmsCEAB-full out of PE3 and pE3R
 #'''UPDATE REGISTRY''': create registry biobrick parts and choose numbers for inverter constructs (w/ and w/out osmY attached) '''as well as all intermediate BGD, IAGD, SAGD parts!!!!''' - p.s. anybody out of town is welcome to help. we seem to be very behind on this.
+#digest J45298 miniprep (from 8/29) with '''XP''' (run a gel to make sure it looks correctly mutagenized)
+#miniprep iGEM yeast shuttle vector and digest it appropriately (check the MCS, or Veena took notes and might know). Also digest our BGD (J45200,J45220) appropriately to insert at the MCS
+#primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29)
+#check if the 0-Q-199A forward sequencing is in from the re-run and see if it looks good
+#glycerol/miniprep/sequence J45396
+#glycerol new Q119
+#pellet and resuspend yeast cells in milk and find a good bread recipe
+#3-part assemble rbss to pchBA.15-muts and transform into top10
+#put our BGD into yeast shuttle vector and transform into top10
+#consider transforming O-Q-119 and O-Q-199 (?) into IK to smell test
+#pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs
+#LC whatever we need for a osmY-Q0440-E0840 plate reader test (and sign up for the block)