IGEM:MIT/2006/Notebook/2006-8-30: Difference between revisions
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#pellet and resuspend yeast cells in milk and find a good bread recipe | #pellet and resuspend yeast cells in milk and find a good bread recipe | ||
#3-part assemble rbss to pchBA.15-muts and transform into top10 | #3-part assemble rbss to pchBA.15-muts and transform into top10 | ||
# | #ligate BGD into yeast shuttle vector and transform into top10 | ||
#consider transforming O-Q-119 and O-Q-199 (?) into IK to smell test | #consider transforming O-Q-119 and O-Q-199 (?) into IK to smell test | ||
#pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs | #pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs | ||
#LC whatever we need for | #LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block) | ||
#smell the pseudomonas with methyl salicylate to see if it was degraded by the cells | |||
==help== | ==help== | ||
#'''UPDATE REGISTRY''': create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) '''as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!!''' - p.s. anybody out of town is welcome to help. we seem to be very behind on this. | #'''UPDATE REGISTRY''': create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) '''as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!!''' - p.s. anybody out of town is welcome to help. we seem to be very behind on this. | ||
Revision as of 19:46, 29 August 2006
to do
- digest J45298 miniprep (from 8/29) with XP (run a gel to make sure it looks correctly mutagenized)
- miniprep iGEM yeast shuttle vector and digest it appropriately (check the MCS, or Veena took notes and might know). Also digest our BGD (J45200,J45220) appropriately to insert at the MCS
- primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29)
- check if the 0-Q-199A forward sequencing is in from the re-run and see if it looks good
- glycerol/miniprep/sequence J45396
- glycerol new Q119
- pellet and resuspend yeast cells in milk and find a good bread recipe
- 3-part assemble rbss to pchBA.15-muts and transform into top10
- ligate BGD into yeast shuttle vector and transform into top10
- consider transforming O-Q-119 and O-Q-199 (?) into IK to smell test
- pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs
- LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block)
- smell the pseudomonas with methyl salicylate to see if it was degraded by the cells
help
- UPDATE REGISTRY: create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!! - p.s. anybody out of town is welcome to help. we seem to be very behind on this.
