IGEM:MIT/2006/Notebook/2006-8-30: Difference between revisions

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==to do==
==to do==
#'''UPDATE REGISTRY''': create registry biobrick parts and choose numbers for inverter constructs (w/ and w/out osmY attached) '''as well as all intermediate BGD, IAGD, SAGD parts!!!!''' - p.s. anybody out of town is welcome to help. we seem to be very behind on this.
#digest J45298 miniprep (from 8/29) with '''XP''' (run a gel to make sure it looks correctly mutagenized) '''-done'''
#digest J45298 miniprep (from 8/29) with '''XP''' (run a gel to make sure it looks correctly mutagenized)
#glycerol'''-done'''/miniprep '''-done'''/sequence J45396 '''-done'''
#miniprep iGEM yeast shuttle vector and digest it appropriately (check the MCS, or Veena took notes and might know). Also digest our BGD (J45200,J45220) appropriately to insert at the MCS
#glycerol new Q119 '''-done'''
#primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29)
#sequence osmY (SO and SN) constructs in bright green box b/c smell test data is not on wiki '''-done'''
#check if the 0-Q-199A forward sequencing is in from the re-run and see if it looks good
#resequence 0-Q-199A in case rerun doesn't work '''-done'''
#glycerol/miniprep/sequence J45396  
#3-part assemble rbss to pchBA.15-muts and transform into top10 '''-done'''
#glycerol new Q119
#transform O-Q-119 and O-Q-199 (?) into IK to smell test '''-done'''
#pellet and resuspend yeast cells in milk and find a good bread recipe
#pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs '''-done, but repeated  the ligation/transformation due to 2XYT contamination'''
#3-part assemble rbss to pchBA.15-muts and transform into top10
#LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block) '''-done'''
#put our BGD into yeast shuttle vector and transform into top10
#smell the pseudomonas cultures (with added methyl salicylate) to see if their minty-ness degraded overnight -'''done'''
#consider transforming O-Q-119 and O-Q-199 (?) into IK to smell test
#set up a new pseudomonas test '''-done'''
#pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs
#LC whatever we need for a osmY-Q0440-E0840 plate reader test (and sign up for the block)

Latest revision as of 14:43, 30 August 2006

to do

  1. digest J45298 miniprep (from 8/29) with XP (run a gel to make sure it looks correctly mutagenized) -done
  2. glycerol-done/miniprep -done/sequence J45396 -done
  3. glycerol new Q119 -done
  4. sequence osmY (SO and SN) constructs in bright green box b/c smell test data is not on wiki -done
  5. resequence 0-Q-199A in case rerun doesn't work -done
  6. 3-part assemble rbss to pchBA.15-muts and transform into top10 -done
  7. transform O-Q-119 and O-Q-199 (?) into IK to smell test -done
  8. pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs -done, but repeated the ligation/transformation due to 2XYT contamination
  9. LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block) -done
  10. smell the pseudomonas cultures (with added methyl salicylate) to see if their minty-ness degraded overnight -done
  11. set up a new pseudomonas test -done
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