IGEM:MIT/2006/Notebook/2006-8-30: Difference between revisions
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#digest J45298 miniprep (from 8/29) with '''XP''' (run a gel to make sure it looks correctly mutagenized) | #digest J45298 miniprep (from 8/29) with '''XP''' (run a gel to make sure it looks correctly mutagenized) '''-done''' | ||
#glycerol'''-done'''/miniprep '''-done'''/sequence J45396 '''-done''' | |||
#glycerol new Q119 '''-done''' | |||
# | #sequence osmY (SO and SN) constructs in bright green box b/c smell test data is not on wiki '''-done''' | ||
#resequence 0-Q-199A in case rerun doesn't work '''-done''' | |||
#glycerol new Q119 | #3-part assemble rbss to pchBA.15-muts and transform into top10 '''-done''' | ||
# | #transform O-Q-119 and O-Q-199 (?) into IK to smell test '''-done''' | ||
#3-part assemble rbss to pchBA.15-muts and transform into top10 | #pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs '''-done, but repeated the ligation/transformation due to 2XYT contamination''' | ||
# | #LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block) '''-done''' | ||
#smell the pseudomonas cultures (with added methyl salicylate) to see if their minty-ness degraded overnight -'''done''' | |||
#pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs | #set up a new pseudomonas test '''-done''' | ||
#LC whatever we need for | |||
Latest revision as of 14:43, 30 August 2006
to do
- digest J45298 miniprep (from 8/29) with XP (run a gel to make sure it looks correctly mutagenized) -done
- glycerol-done/miniprep -done/sequence J45396 -done
- glycerol new Q119 -done
- sequence osmY (SO and SN) constructs in bright green box b/c smell test data is not on wiki -done
- resequence 0-Q-199A in case rerun doesn't work -done
- 3-part assemble rbss to pchBA.15-muts and transform into top10 -done
- transform O-Q-119 and O-Q-199 (?) into IK to smell test -done
- pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs -done, but repeated the ligation/transformation due to 2XYT contamination
- LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block) -done
- smell the pseudomonas cultures (with added methyl salicylate) to see if their minty-ness degraded overnight -done
- set up a new pseudomonas test -done