IGEM:MIT/2006/Notebook/2006-8-30

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(to do)
(to do)
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==to do==
==to do==
#digest J45298 miniprep (from 8/29) with '''XP''' (run a gel to make sure it looks correctly mutagenized) '''-done'''
#digest J45298 miniprep (from 8/29) with '''XP''' (run a gel to make sure it looks correctly mutagenized) '''-done'''
-
#primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29)
+
#glycerol'''-done'''/miniprep '''-done'''/sequence J45396  
-
#check if the 0-Q-199A forward sequencing is in from the re-run and see if it looks good
+
-
#glycerol'''-done'''/miniprep/sequence J45396  
+
#glycerol new Q119 '''-done'''
#glycerol new Q119 '''-done'''
#sequence osmY and R0011 constructs in bright green box b/c smell test data is not on wiki
#sequence osmY and R0011 constructs in bright green box b/c smell test data is not on wiki
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#LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block)
#LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block)
#smell the pseudomonas cultures (with added methyl salicylate) to see if their minty-ness degraded overnight -'''done'''
#smell the pseudomonas cultures (with added methyl salicylate) to see if their minty-ness degraded overnight -'''done'''
 +
 +
 +
==do tomorrow==
 +
#primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29)
 +
#check if the 0-Q-199A forward sequencing is in from the re-run and see if it looks good
==help==
==help==
#'''UPDATE REGISTRY''': create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) '''as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!!''' - p.s. anybody out of town is welcome to help. we seem to be very behind on this.
#'''UPDATE REGISTRY''': create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) '''as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!!''' - p.s. anybody out of town is welcome to help. we seem to be very behind on this.

Revision as of 13:51, 30 August 2006

to do

  1. digest J45298 miniprep (from 8/29) with XP (run a gel to make sure it looks correctly mutagenized) -done
  2. glycerol-done/miniprep -done/sequence J45396
  3. glycerol new Q119 -done
  4. sequence osmY and R0011 constructs in bright green box b/c smell test data is not on wiki
  5. 3-part assemble rbss to pchBA.15-muts and transform into top10
  6. consider transforming O-Q-119 and O-Q-199 (?) into IK to smell test
  7. pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs
  8. LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block)
  9. smell the pseudomonas cultures (with added methyl salicylate) to see if their minty-ness degraded overnight -done


do tomorrow

  1. primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29)
  2. check if the 0-Q-199A forward sequencing is in from the re-run and see if it looks good

help

  1. UPDATE REGISTRY: create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!! - p.s. anybody out of town is welcome to help. we seem to be very behind on this.
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