IGEM:MIT/2006/Notebook/2006-8-30: Difference between revisions
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#3-part assemble rbss to pchBA.15-muts and transform into top10 | #3-part assemble rbss to pchBA.15-muts and transform into top10 | ||
#consider transforming O-Q-119 and O-Q-199 (?) into IK to smell test | #consider transforming O-Q-119 and O-Q-199 (?) into IK to smell test | ||
#pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs | #pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs '''-done''' | ||
#LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block) | #LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block) | ||
#smell the pseudomonas cultures (with added methyl salicylate) to see if their minty-ness degraded overnight -'''done''' | #smell the pseudomonas cultures (with added methyl salicylate) to see if their minty-ness degraded overnight -'''done''' | ||
==do tomorrow== | ==do tomorrow== | ||
Revision as of 11:04, 30 August 2006
to do
- digest J45298 miniprep (from 8/29) with XP (run a gel to make sure it looks correctly mutagenized) -done
- glycerol-done/miniprep -done/sequence J45396
- glycerol new Q119 -done
- sequence osmY and R0011 constructs in bright green box b/c smell test data is not on wiki
- 3-part assemble rbss to pchBA.15-muts and transform into top10
- consider transforming O-Q-119 and O-Q-199 (?) into IK to smell test
- pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs -done
- LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block)
- smell the pseudomonas cultures (with added methyl salicylate) to see if their minty-ness degraded overnight -done
do tomorrow
- primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29)
- check if the 0-Q-199A forward sequencing is in from the re-run and see if it looks good
help
- UPDATE REGISTRY: create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!! - p.s. anybody out of town is welcome to help. we seem to be very behind on this.
