IGEM:MIT/2006/Notebook/2006-8-30

to do

1. digest J45298 miniprep (from 8/29) with XP (run a gel to make sure it looks correctly mutagenized) -done
2. primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29)
3. check if the 0-Q-199A forward sequencing is in from the re-run and see if it looks good
4. glycerol/miniprep/sequence J45396
5. glycerol new Q119
6. pellet and resuspend yeast cells in milk and find a good bread recipe
7. 3-part assemble rbss to pchBA.15-muts and transform into top10
8. ligate BGD into yeast shuttle vector and transform into top10
9. consider transforming O-Q-119 and O-Q-199 (?) into IK to smell test
10. pick multiple colonies from 30.BAT.30.THI3.15 (i.e. J45399) plates and make LCs
11. LC whatever we need for an osmY-Q0440-E0840 plate reader test (and sign up for the block)
12. smell the pseudomonas cultures (with added methyl salicylate) to see if their minty-ness degraded overnight

help

1. UPDATE REGISTRY: create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!! - p.s. anybody out of town is welcome to help. we seem to be very behind on this.