IGEM:MIT/2006/Notebook/2006-8-31: Difference between revisions
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==to do== | ==to do== | ||
#miniprep iGEM yeast shuttle vector and digest it appropriately | #dilute 4 plate reader LCs 1:500 and load w/Barry at 9 am | ||
# | #glycerol (6) J45399 LCs (note: 2XYT problem) | ||
#primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29) | |||
#check if the 0-Q-199A forward sequencing is in from the re-run and also analyze the incoming 12 sample sequencing order (and throw out duplicates/bad ones) | |||
#sequence J45220-C-mut with internal ATF1 primers | |||
#miniprep iGEM yeast shuttle vector and digest it appropriately. Also digest our BGD (J45200,J45220) appropriately to insert it at the MCS | |||
#ligate BGD into yeast shuttle vector and transform into top10 | |||
#pellet with big centrifuge and resuspend yeast cells in milk and find a good bread recipe | #pellet with big centrifuge and resuspend yeast cells in milk and find a good bread recipe | ||
# | #pick colonies from all 8/30 transformant plates | ||
==ongoing help== | |||
#'''UPDATE REGISTRY''': create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) '''as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!!''' - p.s. anybody out of town is welcome to help. we seem to be very behind on this. | |||
Revision as of 13:57, 30 August 2006
to do
- dilute 4 plate reader LCs 1:500 and load w/Barry at 9 am
- glycerol (6) J45399 LCs (note: 2XYT problem)
- primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29)
- check if the 0-Q-199A forward sequencing is in from the re-run and also analyze the incoming 12 sample sequencing order (and throw out duplicates/bad ones)
- sequence J45220-C-mut with internal ATF1 primers
- miniprep iGEM yeast shuttle vector and digest it appropriately. Also digest our BGD (J45200,J45220) appropriately to insert it at the MCS
- ligate BGD into yeast shuttle vector and transform into top10
- pellet with big centrifuge and resuspend yeast cells in milk and find a good bread recipe
- pick colonies from all 8/30 transformant plates
ongoing help
- UPDATE REGISTRY: create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!! - p.s. anybody out of town is welcome to help. we seem to be very behind on this.
