IGEM:MIT/2006/Notebook/2006-8-4: Difference between revisions

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#peoples' schedules
#peoples' schedules


==Transformants LC To Do==
==Colony PCR==
#LC 30.BS.15<sub>A/C</sub> and 30.BS.15<sub>A/T</sub>and 30.BS.15<sub>A</sub> and osmY<sub>A/C, A/T</sub>
#B0030-BSMT-B0015
#*Next step is to cut 30.BS.15 with XP
#B0030-ATF1mut-B0015(2)
#LC 30.ATF.15<sub>A/C</sub> and 30.ATF.15<sub>A/T</sub> and 30.ATF.15<sub>A</sub>
#B0030-ATF1mut-B0015(6)
#*Next step is to cut 30.ATF.15 with XP
 
==Sequencing==
#only sequence promising colony PCR results
#*weed out BOO30 in backbone


==Transformants Found==
==Transformants Found==
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#Since Meagan said that the AC and AT backbones did not work in her assembly and they did not work in our assembly, we should try to get new backbones for the antibiotic construction method.  Until then, we should do assemblies using just the gel extraction method.
#Since Meagan said that the AC and AT backbones did not work in her assembly and they did not work in our assembly, we should try to get new backbones for the antibiotic construction method.  Until then, we should do assemblies using just the gel extraction method.
#We need to check to see if the three plates contain the correct colonies.  We will colony PCR today, and if we see the correct bands, we should LC, miniprep, and sequence those colonies while moving ahead with attaching R0040 to the two structures.
#We need to check to see if the three plates contain the correct colonies.  We will colony PCR today, and if we see the correct bands, we should LC, miniprep, and sequence those colonies while moving ahead with attaching R0040 to the two structures.
==digests (11:45)==
pSB1AT3: EP '''x2'''
THI3: SX

Revision as of 08:52, 4 August 2006

10:30 Team Meeting

  1. LCs to do
  2. colony PCRs for anybody?? or sequencing
  3. plan digests/ligations/etc.
  4. big picture rolls/project PLAN
  5. peoples' schedules

Colony PCR

  1. B0030-BSMT-B0015
  2. B0030-ATF1mut-B0015(2)
  3. B0030-ATF1mut-B0015(6)

Sequencing

  1. only sequence promising colony PCR results
    • weed out BOO30 in backbone

Transformants Found

B0030-ATF1-B0015 2 (Gel Extraction), B0030-ATF1-B0015 6 (Gel Extraction), and B0030-BSMT-B0015 (Gel Extraction)

Conclusions

  1. pUC19 again did not work. We need a new control plasmid.
  2. Since Meagan said that the AC and AT backbones did not work in her assembly and they did not work in our assembly, we should try to get new backbones for the antibiotic construction method. Until then, we should do assemblies using just the gel extraction method.
  3. We need to check to see if the three plates contain the correct colonies. We will colony PCR today, and if we see the correct bands, we should LC, miniprep, and sequence those colonies while moving ahead with attaching R0040 to the two structures.

digests (11:45)

pSB1AT3: EP x2 THI3: SX

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