IGEM:MIT/2006/Notebook/2006-8-7: Difference between revisions

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==Ligations==
==Sequencing==
#Received (6 samples) with some interesting results - hopefully we remedied and will have complete constructs of both ATF1 and BSMT on 8/8 to begin smell testing
#Sent new sequencing order (42 samples) --- remember that the ATF1s probably have double RBS parts!! and that although we sequenced to check, we did not use them in our 8/7 ligations.
 
==Ligations/Transformations into Top10 cells==
#R0040<sub>ES</sub> + A/T<sub>EP</sub> + B0032.J45014.B0015(2)<sub>XP</sub>
#R0040<sub>ES</sub> + A/T<sub>EP</sub> + B0032.J45014.B0015(2)<sub>XP</sub>
#R0040<sub>ES</sub> + A/T<sub>EP</sub> + B0030.J45014.B0015(4)<sub>XP</sub>
#R0040<sub>ES</sub> + A/T<sub>EP</sub> + B0030.J45014.B0015(4)<sub>XP</sub>
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==''SAGD''==
==''SAGD''==
*Tried the pCHB, pCHA and pCHBA PCR again with new pCHBrev primer
*Tried the pchB, pchA and pchBA PCR again with new pchBrev primer
**will run gel to check results on 8/8

Latest revision as of 14:49, 7 August 2006

Sequencing

  1. Received (6 samples) with some interesting results - hopefully we remedied and will have complete constructs of both ATF1 and BSMT on 8/8 to begin smell testing
  2. Sent new sequencing order (42 samples) --- remember that the ATF1s probably have double RBS parts!! and that although we sequenced to check, we did not use them in our 8/7 ligations.

Ligations/Transformations into Top10 cells

  1. R0040ES + A/TEP + B0032.J45014.B0015(2)XP
  2. R0040ES + A/TEP + B0030.J45014.B0015(4)XP
  3. R0040ES + A/TEP + B0030.J45014.B0015(6)XP
  4. R0040ES + A/TEP + B0030.BSMT.B0015(13)XP
  5. R0040ES + A/TEP + B0030.BSMT.B0015(14)XP
  6. R0040ES + A/TEP + B0030.BSMT.B0015(-)XP
  7. R0040ES + A/TEP + B0032.BSMT.B0015(A)XP
  8. R0040ES + A/TEP + B0032.BSMT.B0015(B)XP
  9. osmYSP + B0032.J45014.B0015(2)XP
  10. osmYSP + B0030.J45014.B0015(6)XP
  11. osmYSP + B0030.BSMT.B0015(13)XP
  12. osmYSP + B0030.BSMT.B0015(14)XP
  13. osmYSP + B0030.BSMT.B0015(-)XP
  14. osmYSP + B0032.BSMT.B0015(A)XP
  15. osmYSP + B0032.BSMT.B0015(B)XP

SAGD

  • Tried the pchB, pchA and pchBA PCR again with new pchBrev primer
    • will run gel to check results on 8/8
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