IGEM:MIT/2006/Notebook/2006-8-9: Difference between revisions
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#check 42 sample sequencing order in VectorNTI | #check 42 sample sequencing order in VectorNTI | ||
#*remember ATF1 parts prob have 2 RBS | #*remember ATF1 parts prob have 2 RBS | ||
#SMELL LCs (and miniprep | #SMELL LCs (and miniprep plus sequence promising ones) | ||
#*remember that the ATF1 assembly is a mutant version | |||
#ATF1 mutagenesis (??) | |||
#make LCs of pUCP22 cell colonies (LB Amp) | #make LCs of pUCP22 cell colonies (LB Amp) | ||
#make LCs of overnight transformants (all in LB A/T, some with SA or IA) | #make LCs of overnight transformants (all in LB A/T, some with SA or IA) | ||
Revision as of 13:48, 8 August 2006
To do
- check 42 sample sequencing order in VectorNTI
- remember ATF1 parts prob have 2 RBS
- SMELL LCs (and miniprep plus sequence promising ones)
- remember that the ATF1 assembly is a mutant version
- ATF1 mutagenesis (??)
- make LCs of pUCP22 cell colonies (LB Amp)
- make LCs of overnight transformants (all in LB A/T, some with SA or IA)
