IGEM:MIT/2006/Notebook/2006-8-9

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==Smells!!!! : )==
==Smells!!!! : )==
*the banana and wintergreen generating devices are assembled and WORKING!!! YAY!!! what an exciting day :-D
*the banana and wintergreen generating devices are assembled and WORKING!!! YAY!!! what an exciting day :-D
 +
 +
==Our New Biobricks!==
 +
Let's give a big warm welcome to:
 +
#J45100 (Prom40+RBS30+BSMT+Term15)
 +
#J45120 (Prom40+RBS32+BSMT+Term15)
 +
#J45170 (PromOS+RBS32+BSMT+Term15)
 +
#J45200 (Prom40+RBS30+ATF1+Term15)
 +
#J45220 (Prom40+RBS32+ATF1+Term15)
 +
 +
We will also be updating the registry with all of our intermediate parts as well. Here's a quick outline of our planned number scheme:
 +
 +
#J4510x (Prom40+RBS30+BSMT+Term15) and all precursors
 +
#J4512x (Prom40+RBS32+BSMT+Term15) and all precursors
 +
#J4515x (PromOS+RBS30+BSMT+Term15) and all precursors
 +
#J4517x (PromOS+RBS32+BSMT+Term15) and all precursors
 +
#J4520x (Prom40+RBS30+ATF1+Term15) and all precursors
 +
#J4522x (Prom40+RBS32+ATF1+Term15) and all precursors
 +
#J4525x (PromOS+RBS30+ATF1+Term15) and all precursors
 +
#J4527x (PromOS+RBS32+ATF1+Term15) and all precursors
 +
 +
J453xx, 4xx etc will be for the next devices we build.
==To do==
==To do==

Revision as of 11:35, 9 August 2006

Smells!!!! : )

  • the banana and wintergreen generating devices are assembled and WORKING!!! YAY!!! what an exciting day :-D

Our New Biobricks!

Let's give a big warm welcome to:

  1. J45100 (Prom40+RBS30+BSMT+Term15)
  2. J45120 (Prom40+RBS32+BSMT+Term15)
  3. J45170 (PromOS+RBS32+BSMT+Term15)
  4. J45200 (Prom40+RBS30+ATF1+Term15)
  5. J45220 (Prom40+RBS32+ATF1+Term15)

We will also be updating the registry with all of our intermediate parts as well. Here's a quick outline of our planned number scheme:

  1. J4510x (Prom40+RBS30+BSMT+Term15) and all precursors
  2. J4512x (Prom40+RBS32+BSMT+Term15) and all precursors
  3. J4515x (PromOS+RBS30+BSMT+Term15) and all precursors
  4. J4517x (PromOS+RBS32+BSMT+Term15) and all precursors
  5. J4520x (Prom40+RBS30+ATF1+Term15) and all precursors
  6. J4522x (Prom40+RBS32+ATF1+Term15) and all precursors
  7. J4525x (PromOS+RBS30+ATF1+Term15) and all precursors
  8. J4527x (PromOS+RBS32+ATF1+Term15) and all precursors

J453xx, 4xx etc will be for the next devices we build.

To do

  1. check 42 sample sequencing order in VectorNTI
    • remember ATF1 parts prob have 2 RBS
  2. SMELL LCs (and miniprep plus sequence promising ones)
    • remember that the ATF1 assembly is a mutant version
  3. ATF1 mutagenesis (??)
  4. make LCs of pUCP22 cell colonies (LB Amp)
  5. make LCs of overnight transformants (all in LB A/T, some with SA or IA)
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