IGEM:MIT/2006/Notebook/2006-9-1: Difference between revisions
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#digest: J45299 (3) and J45319 (3) with '''XP''' | #digest: J45299 (3) and J45319 (3) with '''XP''' | ||
#*run a gel | #*run a gel | ||
#sequence: | #sequence: (24 wells) | ||
#*J45220-C-mut with ATF1 primers | #*J45220-C-mut with ATF1 primers | ||
#*all other minipreps (except pE3) | #*all other minipreps (except pE3) | ||
Revision as of 16:44, 31 August 2006
to do
- glycerol: all LCs
- start a smell test: add precursor to the LCs of 0-Q-119 and O-Q-199
- am miniprep (12): J45397 (1), J45398 (1), J45399 (3), J45299 (3), J45319 (3), pE3 (1)
- note: I don't think that the yeast will be grown up enough to miniprep this early in the day
- analyze: incoming 20 sample sequencing order
- throw out bad sequences
- digest: J45299 (3) and J45319 (3) with XP
- run a gel
- sequence: (24 wells)
- J45220-C-mut with ATF1 primers
- all other minipreps (except pE3)
- PCR: pmsCEAB out of pE3(new) and pE3R
- adjust annealing time, check GC content, ask advisor advice on PCRing large coding regions
- clean up??
- run a gel
- graph the plate reader data
- pellet: yeast cells in big centrifuge, resuspend in milk, try baking
- ask samantha for help
- pm miniprep: iGEM yeast
- follow yeast miniprep protocol, vortex cells first, ask samantha for help
