IGEM:MIT/2006/Notebook/2006-9-1: Difference between revisions

Latest revision as of 12:01, 1 September 2006

glycerol: all LCs -done
start a smell test: add precursor to the LCs of 0-Q-119 and O-Q-199 -done, however, results don't look good
am miniprep (12): J45397 (1), J45398 (1), J45399 (3), J45299 (3), J45319 (3), pE3 (1) -done
analyze: incoming 20 sample sequencing order -done
- throw out bad sequences -done
digest: J45299 (3) and J45319 (3) with XP -done
- run a gel
sequence: (32 wells) -done
- J45220-C-mut with ATF1 primers -done
- all other minipreps (except pE3) -done
- J45398-no mut: 3,4,6,7 -done
PCR: pmsCEAB out of pE3(new) and pE3R -done
- adjust annealing time, check GC content, ask advisor advice on PCRing large coding regions -done
- clean up??
- run a gel
graph the plate reader data (call stephen)
3-part ligate/transform into A/C backbone:
- J45299:XP with R0011:ES
- J45319:XP with R0011:ES
pellet: yeast cells in big centrifuge, resuspend in milk, try baking
- ask samantha for help
pm miniprep: iGEM yeast
- follow yeast miniprep protocol, vortex cells first, ask samantha for help
- we can do XS digest/freeze of yeast miniprep on monday and ligate/transform with ATF1 when samantha is around and when we have tried out the bread machine

@@ Line 1: / Line 1: @@
 ==to do==
-#analyze 20 sample sequencing order
+#glycerol: all LCs '''-done'''
+#start a smell test: add precursor to the LCs of 0-Q-119 and O-Q-199 '''-done, however, results don't look good'''
+#am miniprep (12): J45397 (1), J45398 (1), J45399 (3), J45299 (3), J45319 (3), pE3 (1) '''-done'''
+#analyze: incoming 20 sample sequencing order '''-done'''
+#*throw out bad sequences '''-done'''
+#digest: J45299 (3) and J45319 (3) with '''XP''' '''-done'''
+#*run a gel
+#sequence: (32 wells) '''-done'''
+#*J45220-C-mut with ATF1 primers '''-done'''
+#*all other minipreps (except pE3) '''-done'''
+#*J45398-no mut: 3,4,6,7 '''-done'''
+#PCR: pmsCEAB out of pE3(new) and pE3R '''-done'''
+#*adjust annealing time, check GC content, ask advisor advice on PCRing large coding regions '''-done'''
+#*clean up??
+#*run a gel
+#graph the plate reader data (call stephen)
+#3-part ligate/transform into A/C backbone:
+#*J45299:XP with R0011:ES
+#*J45319:XP with R0011:ES
+#pellet: yeast cells in big centrifuge, resuspend in milk, try baking
+#*ask samantha for help
+#pm miniprep: iGEM yeast
+#*follow yeast miniprep protocol, vortex cells first, ask samantha for help
+#*we can do XS digest/freeze of yeast miniprep on monday and ligate/transform with ATF1 when samantha is around and when we have tried out the bread machine