IGEM:MIT/2006/Notebook/2006-9-1: Difference between revisions
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#*J45220-C-mut with ATF1 primers | #*J45220-C-mut with ATF1 primers | ||
#*all other minipreps (except pE3) | #*all other minipreps (except pE3) | ||
#* | #*J45398-no mut: 3,4,6,7 | ||
#PCR: pmsCEAB out of pE3(new) and pE3R | #PCR: pmsCEAB out of pE3(new) and pE3R | ||
#*adjust annealing time, check GC content, ask advisor advice on PCRing large coding regions | #*adjust annealing time, check GC content, ask advisor advice on PCRing large coding regions | ||
Revision as of 11:26, 1 September 2006
to do
- glycerol: all LCs
- start a smell test: add precursor to the LCs of 0-Q-119 and O-Q-199
- am miniprep (12): J45397 (1), J45398 (1), J45399 (3), J45299 (3), J45319 (3), pE3 (1)
- note: I don't think that the yeast will be grown up enough to miniprep this early in the day
- analyze: incoming 20 sample sequencing order
- throw out bad sequences
- digest: J45299 (3) and J45319 (3) with XP
- run a gel
- sequence: (32 wells)
- J45220-C-mut with ATF1 primers
- all other minipreps (except pE3)
- J45398-no mut: 3,4,6,7
- PCR: pmsCEAB out of pE3(new) and pE3R
- adjust annealing time, check GC content, ask advisor advice on PCRing large coding regions
- clean up??
- run a gel
- graph the plate reader data (call stephen)
- 3-part ligate/transform into A/C backbone:
- J45299:XP with R0011:ES
- J45319:XP with R0011:ES
- need to find more backbone!!
- pellet: yeast cells in big centrifuge, resuspend in milk, try baking
- ask samantha for help
- pm miniprep: iGEM yeast
- follow yeast miniprep protocol, vortex cells first, ask samantha for help
- we can do XS digest/freeze of yeast miniprep on monday and ligate/transform with ATF1 when samantha is around and when we have tried out the bread machine
