IGEM:MIT/2006/Notebook/2006-9-12: Difference between revisions
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#*7-9 = osmY-E0840 FN amp | #*7-9 = osmY-E0840 FN amp | ||
#colony PCR '''lots''' of colonies from the y0078 and y0080 plates: looking for an ATF1 length insert!! '''done: pcr and LCs (SP) 2 gels (KB)''' | #colony PCR '''lots''' of colonies from the y0078 and y0080 plates: looking for an ATF1 length insert!! '''done: pcr and LCs (SP) 2 gels (KB)''' | ||
#sequence analysis '''done: KB''' | |||
#*320s are definately correct | |||
#*all 399s incorrect | |||
#397 definately correct for final BB box | |||
#1148-mutated J45220 definately correct/successful mutagenesis | |||
#NOTE: unfortunately, i don't think that the 6 sample sequence order of the 398-muts was ever completed today. since it is way past 5, i moved this task to tomorrow's wiki (KB) | |||
Revision as of 15:56, 12 September 2006
to do
- plate reader: 7 tubes in 37 room (amp, a/t, a/k backbone controls) done: dilutions (SP), loading at 5pm (KB)
- E 1-3 = J450987 a/k
- E 4-6 = R0040-E0840 amp
- E 7-9 = J45270 a/t
- E 10-12 = osmY-E0840 SN a/t
- F 1-3 = J45017 amp
- F 4-6 = osmY-Q-E0840 FN a/k
- 7-9 = osmY-E0840 FN amp
- colony PCR lots of colonies from the y0078 and y0080 plates: looking for an ATF1 length insert!! done: pcr and LCs (SP) 2 gels (KB)
- sequence analysis done: KB
- 320s are definately correct
- all 399s incorrect
- 397 definately correct for final BB box
- 1148-mutated J45220 definately correct/successful mutagenesis
- NOTE: unfortunately, i don't think that the 6 sample sequence order of the 398-muts was ever completed today. since it is way past 5, i moved this task to tomorrow's wiki (KB)
