IGEM:MIT/2006/Notebook/2006-9-17

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(To do)
Current revision (14:30, 17 September 2006) (view source)
(To do)
 
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*transform mutagenesis (going to have to be creative like i was today: either get new cells from Knight Lab or dig in the temporary freezer upstairs for our box)
*transform mutagenesis (going to have to be creative like i was today: either get new cells from Knight Lab or dig in the temporary freezer upstairs for our box)
*pull out LCs of any good J45014-yeast assemblies from 37 room. make glycerols --(can hopefully miniprep if time)
*pull out LCs of any good J45014-yeast assemblies from 37 room. make glycerols --(can hopefully miniprep if time)
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==Results==
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*No transformants J45120-J45320 (1)
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*No transformants J45120-J45320 (2)
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*No transformants osQ-J45119-J45320 (1)
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*No transformants osQ-J45119-J45320 (2)
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*No transformants omsY-J45219
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 +
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*LCed osmY (FN)-J45199

Current revision

To do

  • make a/c LCs of transformants from yesterday (6 plates)
  • transform mutagenesis (going to have to be creative like i was today: either get new cells from Knight Lab or dig in the temporary freezer upstairs for our box)
  • pull out LCs of any good J45014-yeast assemblies from 37 room. make glycerols --(can hopefully miniprep if time)

Results

  • No transformants J45120-J45320 (1)
  • No transformants J45120-J45320 (2)
  • No transformants osQ-J45119-J45320 (1)
  • No transformants osQ-J45119-J45320 (2)
  • No transformants omsY-J45219


  • LCed osmY (FN)-J45199
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