IGEM:MIT/2006/Notebook/2006-9-25: Difference between revisions

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I am ligating and transforming SAGD.WGD and y0078.atf1, y0080.atf1.  Kate will plate. '''-done'''  Tomorrow, it'd be great if people could come into make liquid cultures and/or colony PCR from plates 7 and 8.
#Stephen ligate and transform SAGD.WGD and y0078.atf1 and y0080.atf1.  Kate plate them: '''-done'''   
 
#Tomorrow, it'd be great if people could come in to make liquid cultures of plates 1, 2, 3, and 4 and/or colony PCR from plates 7 and 8.
 
#Sequencing looked good on the osmY(fn)-BGD, so we can fridge plates 5, 6
#*should Make LCs of osmY(fn)-BGD A and B with substrate to be sure smell good

Revision as of 22:53, 25 September 2006

  1. Stephen ligate and transform SAGD.WGD and y0078.atf1 and y0080.atf1. Kate plate them: -done
  1. Tomorrow, it'd be great if people could come in to make liquid cultures of plates 1, 2, 3, and 4 and/or colony PCR from plates 7 and 8.
  1. Sequencing looked good on the osmY(fn)-BGD, so we can fridge plates 5, 6
    • should Make LCs of osmY(fn)-BGD A and B with substrate to be sure smell good