IGEM:MIT/2006/Notebook/2006-9-25: Difference between revisions
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#Stephen ligate and transform SAGD.WGD and y0078.atf1 and y0080.atf1. Kate plate them: '''-done''' | #Stephen ligate and transform SAGD.WGD and y0078.atf1 and y0080.atf1. Kate plate them: '''-done''' | ||
#Tomorrow, it'd be great if people could come in to make liquid cultures of plates 1, 2, 3, and 4 and/or colony PCR from plates 7 and 8. | #Tomorrow, it'd be great if people could come in to make liquid cultures of plates 1, 2, 3, and 4 and/or colony PCR from plates 7 and 8. '''-done kb, but failed''' | ||
#Sequencing looked good on the osmY(fn)-BGD '''(kb)''', so I say that we can fridge plates 5, 6 for now | #Sequencing looked good on the osmY(fn)-BGD '''(kb)''', so I say that we can fridge plates 5, 6 for now | ||
#*should Make LCs of already good sequenced osmY(fn)-BGD A and B with substrate to be sure smell good | #*should Make LCs of already good sequenced osmY(fn)-BGD A and B with substrate to be sure smell good '''-done,kb''' | ||
Revision as of 20:43, 6 October 2006
suicide prevention day
- Stephen ligate and transform SAGD.WGD and y0078.atf1 and y0080.atf1. Kate plate them: -done
- Tomorrow, it'd be great if people could come in to make liquid cultures of plates 1, 2, 3, and 4 and/or colony PCR from plates 7 and 8. -done kb, but failed
- Sequencing looked good on the osmY(fn)-BGD (kb), so I say that we can fridge plates 5, 6 for now
- should Make LCs of already good sequenced osmY(fn)-BGD A and B with substrate to be sure smell good -done,kb
