IGEM:MIT/2006/Notebook/2006-9-5

Update

1. Digested the 10 hopefully mutagenized 30.BAT2.30.THI3 structures and ran on gel.

2. Ran the PMS PCR products on a gel and found that the only one with the correct band is the R tube. I will PCR cleanup that and put it into the metal blue holder labeled "PMSR cleaned up."

3. I am assuming that wells E10, E11, and E12 are the osmY.inverter.e0840 structures. I would say that they were moderately successful. At around stationary phase, fluorescence measurements on those wells leveled off, suggesting that no more GFP was being made in stationary phase^. However, for some reason overall GFP production was roughly twice as large as it was for the R0040 and osmY structures. We may want to keep an eye on this, but it seems as if we should move on and see how the osmY.inverter.xxxs and osmY.xxxs work with the GC.

^This actually makes a lot of sense since GFP is a very, very stable protein, a lot, lot less volatile then isoamyl acetate and methyl salicylate.