IGEM:MIT/2006/Notebook/2006-9-7: Difference between revisions
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#nanodrop minipreps + make 12 glycerols '''-done''' | #nanodrop minipreps + make 12 glycerols '''-done''' | ||
#24 sample sequence order '''-done''' | #24 sample sequence order '''-done''' | ||
#make more LCs from the 399:1 and 320:c plates (in A/T) | #make more LCs from the 399:1 (in A/C) and 320:c plates (in A/T) '''-done''' | ||
#make LCs of two yeast YCP vectors (forgot to put them in rotator on 9/6) | #*picked 3 more colonies from each = 6 total | ||
#buy ingredients and bake bread | #make LCs of two yeast YCP vectors (forgot to put them in rotator on 9/6) '''-done''' | ||
#buy ingredients and bake bread '''-done, but the yeast was prob already dead cuz i didn't have milk yesterday. I wanted to test the machine anyways so i ran the recipe. at least we will find out what bad bread looks like! i also regrew the yeast in YEPD so I can try baking again on 9/8 or 9/9 | |||
Latest revision as of 18:38, 7 September 2006
TO DO
- check sequencing -pretty much done, still need to look more closely at the last couple though
- throw out all bad dna and glycerols -done:KB
- miniprep 12 potentially good ones -done
- nanodrop minipreps + make 12 glycerols -done
- 24 sample sequence order -done
- make more LCs from the 399:1 (in A/C) and 320:c plates (in A/T) -done
- picked 3 more colonies from each = 6 total
- make LCs of two yeast YCP vectors (forgot to put them in rotator on 9/6) -done
- buy ingredients and bake bread -done, but the yeast was prob already dead cuz i didn't have milk yesterday. I wanted to test the machine anyways so i ran the recipe. at least we will find out what bad bread looks like! i also regrew the yeast in YEPD so I can try baking again on 9/8 or 9/9
