IGEM:MIT/2006/Notebook/2006-9-8: Difference between revisions

Latest revision as of 10:29, 9 September 2006

ligated J45399 (4 of them...1A, 1B, 1C, and 2A) to R0011, in A/T backbone, to make J45400 in A/T backbone. We still need to check the sequencing of the J45399s to see which ones were right...hopefully one of the 4 I used was correct. there wasn't a lot of R0011, so I chose 1A, 1B, and 1C b/c they looked the best on the gel, and 2A for the heck of it.

transformed osmy+E0840 FN for the plate reader...used an amp plate b/c I wasn't sure what the resistance was.

@@ Line 1: / Line 1: @@
-==to do==
+==Transformation of J45400==
-#check sequences (dye blob re-runs should be in from 9/1 order)
-#glycerol 6 new LCs (3 of the 399-1s and 3 of the 320-Cs)
+*ligated J45399 (4 of them...1A, 1B, 1C, and 2A) to R0011, in A/T backbone, to make J45400 in A/T backbone.  We still need to check the sequencing of the J45399s to see which ones were right...hopefully one of the 4 I used was correct.  there wasn't a lot of R0011, so I chose 1A, 1B, and 1C b/c they looked the best on the gel, and 2A for the heck of it.
-#Y0080 and Y0078 yeast vectors
-#*miniprep
+==Osmy+E0840 for plate reader==
-#*digest  with XS --->'''must do the artic freeze step following digest'''
-#**run results on a gel
+*transformed osmy+E0840 FN for the plate reader...used an amp plate b/c I wasn't sure what the resistance was.
-#*ligate to J45014:XS (already cut and ready to go in corner of digest tray)
-#*transform
-#pmsCEAB
-#*digest with ES (has 4 Pst sites!!)
-#**run results on a gel and clean-up
-#*ligate into a double resistance backbone:ES (make sure different from w-ever our WGD device is living in)
-#*transform
-#pellet new yeast cells (pm) and resuspend in milk -- bake again or bake on saturday