IGEM:MIT/2006/Notebook/2007-1-10: Difference between revisions
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'''Results from Yesterday''' | '''Results from Yesterday''' | ||
Registry Parts- | Registry Parts- J45120, J45200, J45320, and J45700 all grew up on the appropriate plates and did not grow on others. | ||
J45250 grew up on both AMP/TET and AMP/CHL, and J45900 (the Indole-Knockout Strain) grew up on AMP/CHL. | J45250 grew up on both AMP/TET and AMP/CHL, and J45900 (the Indole-Knockout Strain) grew up on AMP/CHL. | ||
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All of Jason's parts did not grow up on AMP, AMP/KAN, AMP/CHL, or AMP/TET. | All of Jason's parts did not grow up on AMP, AMP/KAN, AMP/CHL, or AMP/TET. | ||
'''Today''' | |||
1. Miniprepped- DONE, sequenced- DONE and made glycerols of J45120, J45200, J45320, and J45700- DONE | |||
2. Made KAN, CHL, and TET plates- DONE and streaked Jason's parts on KAN, CHL, and TET plates- DONE | |||
3. Streaked J45180 on AMP/KAN, AMP/CHL, and AMP/TET- DONE and made a glycerol of it- DONE | |||
4. Made LC of J45400- DONE and streaked on AMP/KAN, AMP/CHL, and AMP/TET- DONE | |||
5. Miniprepped J45250- DONE and retransformed onto AMP/CHL- DONE | |||
6. Transformed two J45800 hopefuls- DONE | |||
7. Found Indole-Knockout strain- NOT DONE | |||
'''Mistake- SORRY''' | |||
J45120 showed growth on both plates, and J45250 did not. The good news is that I can just retransform J45120 instead of J45250 tonight and made a glycerol of it. Also, I was able to turn in the sequencing tubes of J45250 by the end of the day. | |||
Latest revision as of 08:20, 11 January 2007
Results from Yesterday
Registry Parts- J45120, J45200, J45320, and J45700 all grew up on the appropriate plates and did not grow on others.
J45250 grew up on both AMP/TET and AMP/CHL, and J45900 (the Indole-Knockout Strain) grew up on AMP/CHL.
J45180 plate- growth on plate and LC of multiple colonies. Also, growth in the LC of the single colony. No growth from plated single colony.
J45400- I have a streak of this part.
All of Jason's parts did not grow up on AMP, AMP/KAN, AMP/CHL, or AMP/TET.
Today
1. Miniprepped- DONE, sequenced- DONE and made glycerols of J45120, J45200, J45320, and J45700- DONE
2. Made KAN, CHL, and TET plates- DONE and streaked Jason's parts on KAN, CHL, and TET plates- DONE
3. Streaked J45180 on AMP/KAN, AMP/CHL, and AMP/TET- DONE and made a glycerol of it- DONE
4. Made LC of J45400- DONE and streaked on AMP/KAN, AMP/CHL, and AMP/TET- DONE
5. Miniprepped J45250- DONE and retransformed onto AMP/CHL- DONE
6. Transformed two J45800 hopefuls- DONE
7. Found Indole-Knockout strain- NOT DONE
Mistake- SORRY
J45120 showed growth on both plates, and J45250 did not. The good news is that I can just retransform J45120 instead of J45250 tonight and made a glycerol of it. Also, I was able to turn in the sequencing tubes of J45250 by the end of the day.
