IGEM:MIT/2006/Notebook/2007-1-10: Difference between revisions
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'''Today''' | '''Today''' | ||
1. Miniprepped, sequenced and made glycerols of J45120, J45200, J45320, and J45700 | 1. Miniprepped- DONE, sequenced- DONE and made glycerols of J45120, J45200, J45320, and J45700- DONE | ||
2. | 2. Made KAN, CHL, and TET plates- DONE and streaked Jason's parts on KAN, CHL, and TET plates- DONE | ||
3. Streaked J45180 on AMP/KAN, AMP/CHL, and AMP/TET | 3. Streaked J45180 on AMP/KAN, AMP/CHL, and AMP/TET- DONE and made a glycerol of it- DONE | ||
4. Made LC of J45400 and streaked on AMP/KAN, AMP/CHL, and AMP/TET | 4. Made LC of J45400- DONE and streaked on AMP/KAN, AMP/CHL, and AMP/TET- DONE | ||
5. Miniprepped J45250 and retransformed onto AMP/CHL | 5. Miniprepped J45250- DONE and retransformed onto AMP/CHL- DONE | ||
6. Transformed J45800 | 6. Transformed two J45800 hopefuls- DONE | ||
7. | 7. Found Indole-Knockout strain- NOT DONE | ||
'''Mistake- SORRY''' | |||
J45120 showed growth on both plates, and J45250 did not. The good news is that I can just retransform J45120 instead of J45250 tonight and made a glycerol of it. Also, I was able to turn in the sequencing tubes of J45250 by the end of the day. | |||
Latest revision as of 08:20, 11 January 2007
Results from Yesterday
Registry Parts- J45120, J45200, J45320, and J45700 all grew up on the appropriate plates and did not grow on others.
J45250 grew up on both AMP/TET and AMP/CHL, and J45900 (the Indole-Knockout Strain) grew up on AMP/CHL.
J45180 plate- growth on plate and LC of multiple colonies. Also, growth in the LC of the single colony. No growth from plated single colony.
J45400- I have a streak of this part.
All of Jason's parts did not grow up on AMP, AMP/KAN, AMP/CHL, or AMP/TET.
Today
1. Miniprepped- DONE, sequenced- DONE and made glycerols of J45120, J45200, J45320, and J45700- DONE
2. Made KAN, CHL, and TET plates- DONE and streaked Jason's parts on KAN, CHL, and TET plates- DONE
3. Streaked J45180 on AMP/KAN, AMP/CHL, and AMP/TET- DONE and made a glycerol of it- DONE
4. Made LC of J45400- DONE and streaked on AMP/KAN, AMP/CHL, and AMP/TET- DONE
5. Miniprepped J45250- DONE and retransformed onto AMP/CHL- DONE
6. Transformed two J45800 hopefuls- DONE
7. Found Indole-Knockout strain- NOT DONE
Mistake- SORRY
J45120 showed growth on both plates, and J45250 did not. The good news is that I can just retransform J45120 instead of J45250 tonight and made a glycerol of it. Also, I was able to turn in the sequencing tubes of J45250 by the end of the day.
