IGEM:MIT/2006/Notebook/2007-1-10

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Results from Yesterday

Registry Parts- J45120, J45200, J45320, and J45700 all grew up on the appropriate plates and did not grow on others.

J45250 grew up on both AMP/TET and AMP/CHL, and J45900 (the Indole-Knockout Strain) grew up on AMP/CHL.

J45180 plate- growth on plate and LC of multiple colonies. Also, growth in the LC of the single colony. No growth from plated single colony.

J45400- I have a streak of this part.

All of Jason's parts did not grow up on AMP, AMP/KAN, AMP/CHL, or AMP/TET.

Today

1. Miniprepped, sequenced and made glycerols of J45120, J45200, J45320, and J45700.

2. Streaked Jason's parts on KAN, CHL, and TET plates.

3. Streaked J45180 on AMP/KAN, AMP/CHL, and AMP/TET

4. Made LC of J45400 and streaked on AMP/KAN, AMP/CHL, and AMP/TET

5. Miniprepped J45250 and retransformed onto AMP/CHL

6. Transformed J45800 #15, J45600 #5, and J45600 #1 onto proper plates

7. Find Indole-Knockout strain

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