IGEM:MIT/2006/Notebook/2007-1-11

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Plating Results from Yesterday

1. Jason's parts finally grew up. The control, B0015, worked only on the Kan plate as expected (it is in the 1AK3 plasmid). Mach I interestingly grew up on Kan and Tet but not Chl. pBANANA #10 and #12 both grew up on Kan and Chl but not on Tet.

2. J45180 only grew up on AMP/KAN as expected, confirming that the cell is unlikely to have plasmids of different resistances in it.

3. Interestingly, J45400, which was streaked out by Meagan on an AMP/KAN plate did not grow on AMP/KAN, AMP/TET, or AMP/CHL. I will repeat this experiment today.

4. Many colonies were seen from the transformations of J45120, the two J45800 hopefuls, and pUC18.

Plan Today

1. Miniprep, glycerol, and sequence J45400

2. Repeat streaking of J45400 onto AMP/KAN, AMP/TET, and AMP/CHL.

3. Perform colony PCR on many colonies from the J45120 and J45800 transformations.

4. Track down Indole-Knockout cells

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