IGEM:MIT/2006/Notebook/2007-1-12

Check out the checklist for each part on Stephen's contributions page to see progress.

J45400 did not grow up on any plate again.

To Do List

1. Run a gel of the colony PCR products of J45800- DONE

2. Take out the LCs of the good colony PCR colonies from J45120- DONE Wait until smell and antibiotic tests are conducted before miniprepping and sequencing- DONE

3. Check sequencing results of J45250 and J45400.

4. Redo antibiotic test of pBANANAs from Jason, J45120, J45400, and controls.

5. Streak J45120, J45200, and J45250 onto plates to conduct smell tests for tomorrow.

6. Streak the plate of J45180 from multiple colonies onto a new plate to find isolated colonies, which can be used for colony PCR tomorrow.

7. Find Indole-Knockout Cells