IGEM:MIT/2006/Notebook/2007-1-13: Difference between revisions
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J45120- All three clones passed the antibiotic test, meaning that we have isolated colonies with just the one plasmid in it. We will smell test tonight and then miniprep tomorrow morning if they work. | J45120- All three clones passed the antibiotic test, meaning that we have isolated colonies with just the one plasmid in it. We will smell test tonight and then miniprep tomorrow morning if they work. | ||
J45180- I am colony PCRing those today. | J45180- I am colony PCRing those today. They will be run on the gel along with the colony PCRs of J45800 from yesterday. | ||
J45200- cells aren't grown up enough to effectively see if they smell like banana. | J45200- cells aren't grown up enough to effectively see if they smell like banana. | ||
J45250- cells smell like banana. YEH!!!! | J45250- cells smell like banana. YEH!!!! | ||
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pBANANA- pBANANA 10 and 12 grows on C and K and not A and T, Mach I grows on T and K and not A and C, controls work. This is the same result as before, and I used different plates. So, it seems that my results have to be correct. I will miniprep these tomorrow to submit for sequencing Monday. | pBANANA- pBANANA 10 and 12 grows on C and K and not A and T, Mach I grows on T and K and not A and C, controls work. This is the same result as before, and I used different plates. So, it seems that my results have to be correct. I will miniprep these tomorrow to submit for sequencing Monday. | ||
J45800- | J45800- These will be run on the gel with the J45180 colony PCRs later in the day. | ||
Revision as of 11:09, 13 January 2007
Results from Yesterday
J45120- All three clones passed the antibiotic test, meaning that we have isolated colonies with just the one plasmid in it. We will smell test tonight and then miniprep tomorrow morning if they work.
J45180- I am colony PCRing those today. They will be run on the gel along with the colony PCRs of J45800 from yesterday.
J45200- cells aren't grown up enough to effectively see if they smell like banana.
J45250- cells smell like banana. YEH!!!!
J45400- still will not grow up on AMP/KAN, AMP/TET, or AMP/CHL but sequencing results are correct.
pBANANA- pBANANA 10 and 12 grows on C and K and not A and T, Mach I grows on T and K and not A and C, controls work. This is the same result as before, and I used different plates. So, it seems that my results have to be correct. I will miniprep these tomorrow to submit for sequencing Monday.
J45800- These will be run on the gel with the J45180 colony PCRs later in the day.
