IGEM:MIT/2006/Notebook/2007-1-14

Results from Colony PCR

J45180- It seems safe to say that this structure is just osmY+inverter-R0040 since we got multiple bands that were merely of this length from multiple colonies which matched the sequencing results. The good news is that I was able to track down a glycerol containing the part osmY.inverter.J45119 (J45170), which allegedly had good sequencing results. I will streak this onto a plate tonight.

J45800- some bands were correct from these colony PCRs. I will miniprep the proper colonies and then run a gel of the DNA. Then, I can gel extract the proper bands.

Results from Smell Test

J45200 and J45250 smell like bananas. The three J45120 cultures smell like mint.

What to do:

J45120- miniprep cultures today for sequencing Tuesday.- DONE See J45400.

J45180- ask Reshma about where she obtained J45180.- DONE In the meantime, streak J45170 onto a plate.

J45200- smells! See J45400

J45250- smells! See J45400

J45320- correct. Wait until J45180 works until connecting. See J45400.

J45400- ask for top 10 competent cells. Whenever you obtain them, you can connect J45400 to J45200 and J45250. In the meantime, you can digest all of them. The same is true for J45120 and J45320 after J45120 is miniprepped.

pBANANA- miniprep cultures tomorrow for sequencing Tuesday.

J45800- I will miprep the proper colonies- DONE and then run a gel of the DNA. Then, I can gel extract the proper bands.