IGEM:MIT/2006/Notebook/2007-1-15: Difference between revisions
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''To do'' | ''To do'' | ||
1. Run gel of cut (hopeful) J45800. | 1. Run gel of cut (hopeful) J45800.- DONE | ||
2. Gel extract J45800. | 2. Gel extract J45800.- DONE | ||
3. Obtain competent top 10 cells. | 3. Obtain competent top 10 cells.- DONE | ||
4. Ligate J45120 to J45320, J45200 and J45250 to J45400, and J45800 to itself. | 4. Ligate J45120 to J45320, J45200 and J45250 to J45400, and J45800 to itself.- DONE | ||
5. Transform ligation mixes. | 5. Transform ligation mixes.- DONE | ||
6. Make an LC of the osmY+inverter+J45119 structure (which passed the antibiotic test last night) | 6. Make an LC of the osmY+inverter+J45119 structure (which passed the antibiotic test last night)- DONE | ||
7. Miniprep pBANANA LCs. | 7. Miniprep pBANANA LCs.- DONE | ||
8. | 8. Make glycerols of pBANANA LCs.- DONE | ||
9. Make a google doc of updates on each part. | 9. Continue search for Indole-Knockout cells.- STILL LOOKING | ||
10. Try to streak out Indole-Knockout cells from paper with controls on LB plate.- DONE | |||
11. Make a google doc of updates on each part. | |||
'''PAPER NOTES''' | |||
*confirm final system | |||
*Quantify at device level and system level | |||
*odorant does-response curve | |||
*Audience: Nature Biotechnology? | |||
*Angle: The assembly process [given a problem, estimate (1) cost (2) time (3) probability of success (4) level of expertise. start w/ estimates from biotech experts; keep in mind that this a data point on the road to our eventual goal] | |||
**I don't understand what this "Angle" statement is saying. AG | |||
Latest revision as of 13:04, 16 January 2007
To do
1. Run gel of cut (hopeful) J45800.- DONE
2. Gel extract J45800.- DONE
3. Obtain competent top 10 cells.- DONE
4. Ligate J45120 to J45320, J45200 and J45250 to J45400, and J45800 to itself.- DONE
5. Transform ligation mixes.- DONE
6. Make an LC of the osmY+inverter+J45119 structure (which passed the antibiotic test last night)- DONE
7. Miniprep pBANANA LCs.- DONE
8. Make glycerols of pBANANA LCs.- DONE
9. Continue search for Indole-Knockout cells.- STILL LOOKING
10. Try to streak out Indole-Knockout cells from paper with controls on LB plate.- DONE
11. Make a google doc of updates on each part.
PAPER NOTES
- confirm final system
- Quantify at device level and system level
- odorant does-response curve
- Audience: Nature Biotechnology?
- Angle: The assembly process [given a problem, estimate (1) cost (2) time (3) probability of success (4) level of expertise. start w/ estimates from biotech experts; keep in mind that this a data point on the road to our eventual goal]
- I don't understand what this "Angle" statement is saying. AG
