IGEM:MIT/2006/Notebook/2007-1-15: Difference between revisions
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8. Make glycerols of pBANANA LCs.- DONE | 8. Make glycerols of pBANANA LCs.- DONE | ||
9. Continue search for Indole-Knockout | 9. Continue search for Indole-Knockout cells.- STILL LOOKING | ||
10. Make a google doc of updates on each part. | 10. Try to streak out Indole-Knockout cells from paper with controls on LB plate.- DONE | ||
11. Make a google doc of updates on each part. |
Revision as of 14:34, 15 January 2007
To do
1. Run gel of cut (hopeful) J45800.- DONE
2. Gel extract J45800.- DONE
3. Obtain competent top 10 cells.- DONE
4. Ligate J45120 to J45320, J45200 and J45250 to J45400, and J45800 to itself.- DONE
5. Transform ligation mixes.- DONE
6. Make an LC of the osmY+inverter+J45119 structure (which passed the antibiotic test last night)- DONE
7. Miniprep pBANANA LCs.- DONE
8. Make glycerols of pBANANA LCs.- DONE
9. Continue search for Indole-Knockout cells.- STILL LOOKING
10. Try to streak out Indole-Knockout cells from paper with controls on LB plate.- DONE
11. Make a google doc of updates on each part.