IGEM:MIT/2006/Notebook/2007-1-18

To Do:

1. Submit all 108 sequencing samples- DONE

2. Check sequencing results from 1/17 and 1/18

3. Check antibiotic tests of osmY.E0840 SN, osmY.inverter.E0840 FN, R0040.E0840, B0015 (control), and the indole-knockout cells- DONE

4. Retransform osmY.E0840 FN prep

5. Restreak R0040.E0840, osmY.E0840 SN, osmY.inverter.E0840 FN, and B0015

6. Digest osmY.inverter.J45119 and J45320

7. Ligate osmY.inverter.J45119 to J45320

8. Transform (hopeful) J45800

9. Obtain Top 10 Competent Cells

Results from Antibiotic Tests

R0040.E0840

SUCCESSFUL (grew on AMP and not on AT, AK, or AC)

osmYFN.inverter.E0840

SUCCESSFUL (grew on AK and not on AT or AC)

osmYSN.E0840

SUCCESSFUL (grew on AT and not on AK or AC)

B0015 (Control)

SUCCESSFUL (grew on AK and not on AT or AC)

Indole-Knockout cells

SUCCESSFUL (grew on C but not on A, T, or K, consistent with Yale's listing of the strain as Chloramphenicol resistant)