IGEM:MIT/2006/Notebook/2007-1-19: Difference between revisions
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==Course of Action== | ==Course of Action== | ||
1. Colony PCR J45170.J45320s | 1. Colony PCR J45170.J45320s and J45800 hopefuls | ||
2. Miniprep LCs of R0040.E0840, osmYShort.E0840, and osmYFull.inverter.E0840 | 2. Miniprep LCs of R0040.E0840, osmYShort.E0840, and osmYFull.inverter.E0840 | ||
3. LC J45170.J45320, J45800, osmYShort.inverter.E0840 | 3. LC J45170.J45320, J45800, osmYShort.inverter.E0840, osmYFFull.E0840 | ||
4. Make plates | 4. Make plates | ||
Revision as of 09:18, 19 January 2007
Results from Yesterday's Transformations
osmYFull.E0840
Passed antibiotic resistance test- only grows on AMP/TET
J45800 hopefuls
All grew
osmYShort.inverter.E0840
Some colonies
J45170.J45320
Very few colonies- Maybe 3
Course of Action
1. Colony PCR J45170.J45320s and J45800 hopefuls
2. Miniprep LCs of R0040.E0840, osmYShort.E0840, and osmYFull.inverter.E0840
3. LC J45170.J45320, J45800, osmYShort.inverter.E0840, osmYFFull.E0840
4. Make plates
5. Check sequencing (later tonight)
6. Respond to Jason in the GC lab- DONE (MEETING AT 2 PM ON MONDAY TO LEARN HOW TO USE THE MACHINE)
