IGEM:MIT/2006/Notebook/2007-1-19: Difference between revisions

Revision as of 12:27, 19 January 2007

osmYFull.E0840

Passed antibiotic resistance test- only grows on AMP/TET

J45800 hopefuls

All grew

osmYShort.inverter.E0840

Some colonies

J45170.J45320

Very few colonies- Maybe 3

1. Colony PCR J45170.J45320s and J45800 hopefuls- DONE

2. Miniprep LCs of R0040.E0840, osmYShort.E0840, and osmYFull.inverter.E0840- DONE

3. LC J45170.J45320, J45800, osmYShort.inverter.E0840, osmYFFull.E0840

4. Make plates

5. Check sequencing (results will not be available until later tonight)

6. Respond to Jason in the GC lab- DONE (MEETING AT 2 PM ON MONDAY TO LEARN HOW TO USE THE MACHINE)

7. Smell IK cells- SMELL GREAT TO ME- DONE

8. Update google doc

9. Make a list of needed submissions and resubmissions to the Registry- DONE

J45120

Cells in the Registry have a mix of plasmids. We isolated a colony with just J45120 and have a glycerol available.

J45700

Part in the Registry is merely J45320. After sequencing analysis, we will most likely have a correct glycerol of this part.

J45600, J45800, & J45900 (Constitutive Banana)

We are in the process of building/verifying.

J45995, J45996, and truncated osmY versions of each

We are in the process of building/verifying

@@ Line 36: / Line 36: @@
 . Update google doc
-. Make a list of needed submissions and resubmissions to the Registry
+. Make a list of needed submissions and resubmissions to the Registry- DONE
+==List of Needed Submissions to the Registry==
+'''J45120'''
+Cells in the Registry have a mix of plasmids.  We isolated a colony with just J45120 and have a glycerol available.
+'''J45700'''
+Part in the Registry is merely J45320.  After sequencing analysis, we will most likely have a correct glycerol of this part.
+'''J45600, J45800, & J45900 (Constitutive Banana)'''
+We are in the process of building/verifying.
+'''J45995, J45996, and truncated osmY versions of each'''
+We are in the process of building/verifying