IGEM:MIT/2006/Notebook/2007-1-19: Difference between revisions
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7. Smell IK cells- SMELL GREAT TO ME- DONE | 7. Smell IK cells- SMELL GREAT TO ME- DONE | ||
8. Update google doc | 8. Update google doc- DONE | ||
9. Make a list of needed submissions and resubmissions to the Registry- DONE | 9. Make a list of needed submissions and resubmissions to the Registry- DONE | ||
Revision as of 13:19, 19 January 2007
Results from Yesterday's Transformations
osmYFull.E0840
Passed antibiotic resistance test- only grows on AMP/TET
J45800 hopefuls
All grew
osmYShort.inverter.E0840
Some colonies
J45170.J45320
Very few colonies- Maybe 3
Course of Action
1. Colony PCR J45170.J45320s and J45800 hopefuls- DONE
2. Miniprep LCs of R0040.E0840, osmYShort.E0840, and osmYFull.inverter.E0840- DONE
3. LC J45170.J45320, J45800, osmYShort.inverter.E0840, osmYFFull.E0840
4. Make plates
5. Check sequencing (results will not be available until later tonight)
6. Respond to Jason in the GC lab- DONE (MEETING AT 2 PM ON MONDAY TO LEARN HOW TO USE THE MACHINE)
7. Smell IK cells- SMELL GREAT TO ME- DONE
8. Update google doc- DONE
9. Make a list of needed submissions and resubmissions to the Registry- DONE
List of Needed Submissions to the Registry
J45120
Cells in the Registry have a mix of plasmids. We isolated a colony with just J45120 and have a glycerol available.
J45700
Part in the Registry is merely J45320. After sequencing analysis, we will most likely have a correct glycerol of this part.
J45600, J45800, & J45900 (Constitutive Banana)
We are in the process of building/verifying.
J45995, J45996, and truncated osmY versions of each
We are in the process of building/verifying
