IGEM:MIT/2006/Notebook/2007-1-19: Difference between revisions
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==Course of Action== | ==Course of Action== | ||
1. Colony PCR J45170.J45320s | 1. Colony PCR J45170.J45320s and J45800 hopefuls- DONE | ||
2. Miniprep LCs of R0040.E0840, osmYShort.E0840, and osmYFull.inverter.E0840 | 2. Miniprep LCs of R0040.E0840, osmYShort.E0840, and osmYFull.inverter.E0840- DONE | ||
3. LC J45170.J45320, J45800, osmYShort.inverter.E0840 | 3. LC J45170.J45320, J45800, osmYShort.inverter.E0840, osmYFFull.E0840- DONE | ||
4. Make plates | 4. Make plates- DONE | ||
5. Check sequencing (later tonight) | 5. Check sequencing (results will not be available until later tonight) | ||
6. Respond to Jason in the GC lab- DONE (MEETING AT 2 PM ON MONDAY TO LEARN HOW TO USE THE MACHINE) | 6. Respond to Jason in the GC lab- DONE (MEETING AT 2 PM ON MONDAY TO LEARN HOW TO USE THE MACHINE) | ||
7. Smell IK cells- SMELL GREAT TO ME- DONE | |||
8. Update google doc- DONE | |||
9. Make a list of needed submissions and resubmissions to the Registry- DONE | |||
==List of Needed Submissions to the Registry== | |||
'''J45120''' | |||
Cells in the Registry have a mix of plasmids. We isolated a colony with just J45120 and have a glycerol available. | |||
'''J45180''' | |||
The J45180 in the Registry is merely osmY + inverter - R0040. We have another glycerol containing the correct J45180, osmYQ119B. | |||
'''J45700 & J45900 (Constitutive Banana)''' | |||
The J45700 in the Registry is merely J45320. After an antibiotic test and an analysis using the GC, we can submit these parts. | |||
'''J45600 & J45800''' | |||
We are in the process of building/verifying. | |||
'''J45995, J45996, J45997, & J45998''' | |||
Cells glyceroled and verified via sequencing and antibiotic test | |||
'''J45999''' | |||
We have a glycerol of these in our fridge. The entry in the Registry most likely contains a plasmid since it is chloramphenicol AND ampicillin resistant. | |||
Latest revision as of 13:02, 30 January 2007
Results from Yesterday's Transformations
osmYFull.E0840
Passed antibiotic resistance test- only grows on AMP/TET
J45800 hopefuls
All grew
osmYShort.inverter.E0840
Some colonies
J45170.J45320
Very few colonies- Maybe 3
Course of Action
1. Colony PCR J45170.J45320s and J45800 hopefuls- DONE
2. Miniprep LCs of R0040.E0840, osmYShort.E0840, and osmYFull.inverter.E0840- DONE
3. LC J45170.J45320, J45800, osmYShort.inverter.E0840, osmYFFull.E0840- DONE
4. Make plates- DONE
5. Check sequencing (results will not be available until later tonight)
6. Respond to Jason in the GC lab- DONE (MEETING AT 2 PM ON MONDAY TO LEARN HOW TO USE THE MACHINE)
7. Smell IK cells- SMELL GREAT TO ME- DONE
8. Update google doc- DONE
9. Make a list of needed submissions and resubmissions to the Registry- DONE
List of Needed Submissions to the Registry
J45120
Cells in the Registry have a mix of plasmids. We isolated a colony with just J45120 and have a glycerol available.
J45180
The J45180 in the Registry is merely osmY + inverter - R0040. We have another glycerol containing the correct J45180, osmYQ119B.
J45700 & J45900 (Constitutive Banana)
The J45700 in the Registry is merely J45320. After an antibiotic test and an analysis using the GC, we can submit these parts.
J45600 & J45800
We are in the process of building/verifying.
J45995, J45996, J45997, & J45998
Cells glyceroled and verified via sequencing and antibiotic test
J45999
We have a glycerol of these in our fridge. The entry in the Registry most likely contains a plasmid since it is chloramphenicol AND ampicillin resistant.
