IGEM:MIT/2006/Notebook/2007-1-19

Results from Yesterday's Transformations

osmYFull.E0840

Passed antibiotic resistance test- only grows on AMP/TET

J45800 hopefuls

All grew

osmYShort.inverter.E0840

Some colonies

J45170.J45320

Very few colonies- Maybe 3

1. Colony PCR J45170.J45320s, J45800 hopefuls and osmYShort.inverter.E0840s

2. Miniprep LCs of R0040.E0840, osmYShort.E0840, and osmYFull.inverter.E0840

3. LC J45170.J45320, J45800, osmYShort.inverter.E0840

4. Make plates

5. Check sequencing (later tonight)

6. Respond to Jason in the GC lab- DONE (MEETING AT 2 PM ON MONDAY TO LEARN HOW TO USE THE MACHINE)