IGEM:MIT/2006/Notebook/2007-1-19

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Results from Yesterday's Transformations

osmYFull.E0840

Passed antibiotic resistance test- only grows on AMP/TET

J45800 hopefuls

All grew


osmYShort.inverter.E0840

Some colonies

J45170.J45320

Very few colonies- Maybe 3

Course of Action

1. Colony PCR J45170.J45320s and J45800 hopefuls- DONE

2. Miniprep LCs of R0040.E0840, osmYShort.E0840, and osmYFull.inverter.E0840- DONE

3. LC J45170.J45320, J45800, osmYShort.inverter.E0840, osmYFFull.E0840

4. Make plates

5. Check sequencing (results will not be available until later tonight)

6. Respond to Jason in the GC lab- DONE (MEETING AT 2 PM ON MONDAY TO LEARN HOW TO USE THE MACHINE)

7. Smell IK cells- SMELL GREAT TO ME- DONE

8. Update google doc

9. Make a list of needed submissions and resubmissions to the Registry

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