IGEM:MIT/2006/Notebook/2007-1-21

Results from Smell Test (SP)

J45700A- faint for both large and small cultures

J45700B- not present in large culture and faint in small culture

J45700C- faint for both large and small cultures

J45700D- not present in large culture and faint in small culture

J45700E- strongest in large culture of the 700s, faint in small culture

J45900A- good smell in both large and small cultures

J45900B- good smell in both large and small cultures

J45900C- faint smell in large culture and good smell in small culture

J45900D- good smell in large culture and faint smell in small culture

J45600A- faint smell in both large and small cultures

J45600B- very faint if any smell in both large and small cultures

• Note- J45700 is constitutive, autonomous mint, J45900 is constitutive, autonomous banana, and J45600 is stationary phase sensitive, autonomous banana

• • Veena thought that 700A, 700D, 700E, 900C, and 900D smelled but could not smell the others. However, she said that her nose was runny.

• • • Jason thought that each culture he smelled (900A, 900B, 600A, and 700A) smelled great.

To Do

1. Dilute from two samples of each part type into new, 10 mL cultures. We will use these tomorrow when we are experimenting with the GC. SP picked 700A, 700E, 900A, 900B, 600A, and 600B.- DONE

2. Organize plates.- DONE

3. Clean tubes.- DONE

4. Update google doc.- DONE

5. Submit the sequencing sheet.- DONE

6. Streak indole-knockout strain onto plate (first step towards making the cells competent).- DONE