IGEM:MIT/2006/Notebook/2007-1-23: Difference between revisions
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6. Check sequencing results (probably not in until later tonight) | 6. Check sequencing results (probably not in until later tonight) | ||
==Sequencing Results== | |||
ALL osmY structures had great sequencing results. So, we can go ahead with plate reader experiments on those. | |||
Unfortunately, the phase-sensitive banana and mint autonomous structure's sequencing was terrible. The ones that did come out okay did not line up very well. | |||
Revision as of 14:17, 23 January 2007
GCMS Preparation
1. Order vial tubes/caps- DONE
2. Order p-cymene (isoamyl acetate internal standard)- DONE
3. Keep in contact with Jason as to whether CS2 is acceptable for use with the GC- DONE (CS2 is fine; I will contact Heather to order it)
4. Determine concentration of PCNB (methyl salicylate internal standard)- DONE (I think)
5. Find out if the GC will be ready today for mint samples- DONE (WILL NOT BE READY TODAY)
Immediate Plan
1. Check smell tests- DONE (all three J45170.J45320 structures smell, J45170 with precursor added smells great, controls smell bad)
2. Update google doc- DONE
3. Make Indole-Knockout competent cells- DONE
4. Organize/label new plates- DONE
5. Perform antibiotic test on osmYS.inverter.E0840 hopefuls- DONE
6. Check sequencing results (probably not in until later tonight)
Sequencing Results
ALL osmY structures had great sequencing results. So, we can go ahead with plate reader experiments on those.
Unfortunately, the phase-sensitive banana and mint autonomous structure's sequencing was terrible. The ones that did come out okay did not line up very well.
